Abstract
Bioactive nanosilicates are emerging prominent next generation biomaterials due to their intrinsic functional properties such as advanced biochemical and biophysical cues. Recent studies show interesting dose-dependent effect of fluoride ions on the stem cells. Despite of interesting properties of fluoride ions as well as nanosilicate, there is no reported literature on the effect of fluoride-doped nanosilicates on stem cells. We have systematically evaluated the interaction of fluoride nanosilicate platelets (NS + F) with human dental follicle stem cells (hDFSCs) to probe the cytotoxicity, cellular transport (internalization) and osteogenic differentiation capabilities in comparison with already reported nanosilicate platelets without fluoride (NS − F). To understand the osteoinductive and osteoconductive properties of the nanosilicate system, nanosilicate treated hDFSCs are cultured in three different medium namely normal growth medium, osteoconductive medium, and osteoinductive medium up to 21 d. NS + F treated stem cells show higher ALP activity, osteopontin levels and significant alizarin red staining compared to NS − F treated cells. This study highlights that the particles having fluoride additives (NS + F) aid in enhancing the osteogenic differentiation capabilities of hDFSCs thus potential nanobiomaterial for periodontal bone tissue regeneration.
Highlights
Bioactive nanosilicates are emerging prominent generation biomaterials due to their intrinsic functional properties such as advanced biochemical and biophysical cues; owing to their composition, enhanced surface area and adsorption properties
Though there is a recent study on the effect of Laponite XLG (NS − F; Nanosilicate platelets without fluoride ions) on hBMSC proliferation and differentiation, the effect on the human dental follicle stem cells (hDFSCs) is yet to be examined
In this paper we have systematically evaluated the cytotoxicity and osteogenic differentiation capabilities of fluoride nanosilicate platelets (NS + F) in comparison with already reported nanosilicate platelets without fluoride (NS − F) towards human dental follicle stem cells in presence of different culture condition to understand the Osteocon nductive and Osteoinductive properties of the nanosilicate system and their interaction with hDFSCs
Summary
Bioactive nanosilicates are emerging prominent generation biomaterials due to their intrinsic functional properties such as advanced biochemical and biophysical cues; owing to their composition, enhanced surface area and adsorption properties. In this study we have used fluoride-dope-nanosilicate platelets, Laponite-XL21 (with empirical formula Na+0.7 [(Si8 Mg5.5 Li0.3) O20 (OH)4-x Fx]−0.7, from BYK Additive and Instrument, USA; abbreviated in the text as ‘Nanosilicate-with-Fluoride (NS + F)) as a new material to probe the interaction with stem cells and their effect on the modulation of the osteogenic differentiation. To our knowledge, this is the first time we are reporting a fluoride nanosilicate system which aids in osteoinduction of hDFSCs, resulting in the osteogenic differentiation. In this paper we have systematically evaluated the cytotoxicity and osteogenic differentiation capabilities of fluoride nanosilicate platelets (NS + F) in comparison with already reported nanosilicate platelets without fluoride (NS − F) towards human dental follicle stem cells (hDFSCs) in presence of different culture condition (normal: NCM, Osteoconductive medium: OCM and Osteoinductive medium: OIM) to understand the Osteocon nductive and Osteoinductive properties of the nanosilicate system and their interaction with hDFSCs
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