Abstract
This study aimed to explore the effect of fluoridated toothpastes on biofilm architecture and enamel demineralization in an in vitro biofilm model. Streptococcus mutans was grown on enamel and treated with slurries of commercial toothpastes, containing SnF<sub>2</sub> or NaF. Water and chlorhexidine were used as negative and positive controls, respectively. The developed biofilms were imaged and enamel demineralization was measured. SnF<sub>2</sub> and NaF toothpaste treatments significantly reduced enamel demineralization, but SnF<sub>2</sub> toothpaste was more effective. Only SnF<sub>2</sub> toothpaste and chlorhexidine treatments caused reductions on biofilm mass and thickness. In conclusion, this biofilm model was able to differentiate the effects of the SnF<sub>2</sub> and NaF toothpastes on biofilm architecture and enamel demineralization.
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