Abstract

Aerosolization of mist from metalworking fluids (MWFs) has been well characterized in previous studies. Much less is known about the aerosolization of microorganisms, although airborne microbial exposures at MWF sites have been associated with occupational respiratory symptoms and diseases. In this study, the effects of fluid type, microorganism concentration in the liquid, and the microbial species on the aerosolization of microorganisms from MWFs were tested. Three microorganisms were employed to represent different size and surface characteristics: Bacillus subtilis bacterial endospores (hydrophobic particles with aerodynamic diameter = 0.9 μm), Pseudomonas fluorescens bacterial vegetative cells (hydrophilic, 0.8 μm), and Penicillium melinii fungal spores (hydrophobic, 3.1 μm). The testing was first performed using a Collison nebulizer to aerosolize microorganisms from three fluids: water, semisynthetic MWF, and soluble oil. No significant difference in the aerosolization ratio (microbial concentration...

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