Effect of Feeding Chemically Treated Mustard (Brassica campestris) Straw on Rumen Fibre Degrading Enzymes in Sheep

S Vaithiyanathan,S K S Raghuvanshi,A S Mishra,R Prasad,A K Misra,R C Jakhmola,M K Tripathi

doi:10.5713/ajas.2003.1610

Abstract

The aim of the experiment was to study the changes in the activities of various rumen fibre degrading enzymes due to the feeding of chemically treated mustard (Brassica campestris) straw in sheep. Mustard straw (MS) (<5 cm particle size) was treated either with urea (4% (w/w), or with 2% sodium hydroxide (NaOH), or with alkaline hydrogen peroxide (2% NaOH and 1.5% hydrogen peroxide (H2O2)) and/or supplemented with 2% (w/w) urea. Seven maintenance type rations were prepared using MS (70 parts) with molasses (5 parts) and concentrate (25 parts). They were untreated MS (CMS), urea treated MS (UMS), urea supplemented MS (MS- US), alkali treated MS (AMS), alkali treated and urea supplemented MS (AMS-US), alkali H2O2 treated MS (AHMS) and alkali H2O2 treated and urea supplemented MS (AHMS-US). They were then compressed into a complete feed block with the help of block making machine. Forty two male hoggets of Malpura breed sheep were equally distributed into each treatment group and (were) offered feed and water ad libitum. At the end of 21 days of feeding trial, rumen liquor was collected through stomach tube from three animals in each group at 0 h, 4 h, 8 h, 12 h of post feeding. Results showed that the level of enzyme varied from 8.52 to 11.12, 40.85 to 50.37, 3.22 to 3.78, 2.09 to 2.77 and 31.44 to 44.24 units/100 ml SRL respectively for carboxymethyl cellulase (CMCase), α-amylase, microcrystalline cellulase (MCCase), filter paper (FP) degrading enzyme and α-glucosidase. Processing of MS affected the enzyme activities, in a way, that NaOH and AHP treatment significantly reduced CMCase and FP degrading enzyme. The effect of urea treatment showed an increase in the activity of MCCase and α-glucosidase. But the supplementation of urea increased the activity of CMCase, FP degrading enzyme and α-glucosidase. The CMCase, α-amylase, α-glucosidase activities were highest at 4hr whereas MCCase and FP degrading enzyme had maximum activities at 12 h post feeding Results suggested that MS might need longer time in the rumen for its effective degradation. (Asian-Aust. J. Anim. Sci. 2003. Vol 16, No. 11 : 1610-1613)

Highlights

In arid and semi-arid condition, non-availability of sufficient quantity of feed and fodder necessitates the identification of newer sources of feeds
Dry matter intake of animals ranged from 827 g to 950 g per day amounting to 3.23 to 3.75% of body weight (Table 2) and considered optimum for adult sheep (Misra et al, 2000)
The activities of carboxymethyl cellulase (CMCase), αamylase and filter paper (FP) degrading enzyme in the strained rumen liquor (SRL) of sheep fed with chemically treated mustard straw (MS) containing complete feed block showed significant (p

Summary

INTRODUCTION

In arid and semi-arid condition, non-availability of sufficient quantity of feed and fodder necessitates the identification of newer sources of feeds. To improve the access of the rumen microbial hydrolytic enzyme to cellulose present inside the cell wall constituents, attempts are being made to disrupt the lignocellulose complex through physical/chemical/. The understanding of the status of ruminal microbial fibre degrading enzyme activity would reflect quantitatively the presence of fibre degrading microbes in the rumen (Silva et al, 1987) and the fibre degradation inside the rumen. In this experiment, we intended to study the changes in the activities of various fibre degrading enzymes due to various chemical treatment of mustard straw (MS) in sheep

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION