Abstract

In this study, Fe3O4 was added to the anaerobic fermentation process of waste cooking oil (WCO) and aerobic sludge (AS), and its effects on the production of volatile fatty acids (VFAs) were explored from the perspectives of propionic acid concentration, Fe2+ concentration, and microorganisms. The results showed that when the dosage of Fe3O4 was 5 g/L, it was conducive to the hydrolysis and acidification of WCO. And it had a significant effect on the concentration of VFAs and propionic acid. The maximum concentration of VFAs was 7142.84 ± 201.48 mg COD/L, and the maximum concentration of propionic acid was 4990.92 ± 124.76 mg COD/L, which increased by 20.65 % and 27.65 % respectively compared with the blank group. Meanwhile, microbial community analysis showed that Firmicutes, Proteobacteria, and Bacteroidetes are the main phylum for the production of VFA in the anaerobic fermentation of WCO and as. Veillonellaceae under Firmicutes was the main microorganism to produce propionic acid. It provides microbial support for the hydrolysis of WCO and the subsequent production of propionic acid. This study provides a new idea for Fe3O4 to promote the hydrolysis of WCO and produce propionic acid, and brings potential economic benefits to WCO treatment.

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