Effect of Fe nanoparticle on growth and glycolipid biosurfactant production under solid state culture by marine Nocardiopsissp. MSA13A

George Seghal Kiran,Joseph Selvin,Lipton Anuj Nishanth,Kumar Anitha,Sethu Priyadharshini

doi:10.1186/1472-6750-14-48

George Seghal Kiran, Joseph Selvin + Show 3 more

Open Access

https://doi.org/10.1186/1472-6750-14-48

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Journal: BMC Biotechnology	Publication Date: May 21, 2014
Citations: 84	License type: cc-by

Affiliation: Pondicherry University

Abstract

BackgroundIron is an essential element in several pathways of microbial metabolism, and therefore low iron toxicity is expected on the usage of Fe nanoparticles (NPs). This study aims to determine the effect of Fe NPs on biosurfactant production by marine actinobacterium Nocardiopsis sp. MSA13A under solid state culture. Foam method was used in the production of Fe NPs which were long and fiber shaped in nature.ResultsThe SEM observation showed non toxic nature of Fe NPs as no change in the morphology of the filamentous structure of Nocardiopsis MSA13A. The production of biosurfactant by Nocardiopsis MSA13A under solid state culture supplemented with Fe NPs increased to 80% over control. The biosurfactant produced by Nocardiopsis MSA13A was characterized as glycolipid derivative which effectively disrupted the pre-formed biofilm of Vibrio pathogen.ConclusionThe use of metal NPs as supplement would reduce the impact of non-metallic ions of the metal salts in a fermentation process. This would ultimately useful to achieve greener production process for biosurfactants. The present results are first report on the optimization of biosurfactant production under SSC using Fe NPs.

Highlights

Iron is an essential element in several pathways of microbial metabolism, and low iron toxicity is expected on the usage of Fe nanoparticles (NPs)
The present study aims to determine the effect of Fe NPs on biosurfactant production by marine actinobacterium under solid state culture (SSC)
Isolation and identification of biosurfactant producer MSA13A This report is an outcome of bioprospecting of actinobacteria associated with marine sponge Dendilla nigra

Summary

Introduction

Iron is an essential element in several pathways of microbial metabolism, and low iron toxicity is expected on the usage of Fe nanoparticles (NPs). This study aims to determine the effect of Fe NPs on biosurfactant production by marine actinobacterium Nocardiopsis sp. Development of economical engineering processes and the use of low cost feedstock for microorganism growth and surfactant production could reduce the production cost [2]. It is a well-known fact that microorganisms are immensely affected by conditions that prevail surrounding them. Metal supplementation was reported to be one of the critical factors required for enhanced production of biosurfactants [3]. Fe is the key microelement for biosurfactant production in several microorganisms [4]

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