Abstract
The ointment of plastrum testudinis was extracted using petroleum ether, ether and dichloromethane sequentially and the extracts were methyl-esterified. The effects on the proliferation of bone marrow mesenchymal stem cells (bMMSCs) were examined by MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay and flow cytometry analysis. The volatile components of the samples were studied by gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC). The results show that the methyl-esterified parts can promote the proliferation of stem cells and they all contain palmitic acid methyl ester. Palmitic acid methyl ester can promote proliferation when the concentration was 0.15 μg/μL. It may be concluded that the palmitic acid methyl ester is important for the methyl-esterified parts that have effects on proliferation.
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