Abstract
Three enzymes engaged in complex lipid synthesis, diglyceride acyltransferase, cholinephosphotransferase, and lysolecithin acyltransferase were studied in nonfasting hamster intestinal mucosa. Hamsters eating a fat-supplemented diet were compared to those eating a low fat (chow) diet. In villous tips, fat supplementation had no effect on diglyceride acyltransferase or lysolecithin acyltransferase specific activity in the most proximal intestine. Both enzymes demonstrated increased activity in villous tips in response to fat feeding in the distal 3/4 of the gut. Cholinephosphotransferase activity was increased by fat feeding throughout the intestine in villous tips. Diglyceride acyltransferase and lysolecithin acyltransferase activity was greater in villous tips as compared to crypts. Cholinephosphotransferase activity was the same in crypts as villous tips. Lysolecithin acyltransferase was clearly more responsive to fat feeding than was cholinephosphotransferase. These data, in toto, lend further support to the thesis that the lysolecithin acyltransferase pathway provides the lecithin associated with intestinal lipoprotein formation. Of four microsomal enzymes not related to lipid metabolism, only one, NADPH-cytochrome c reductase, was affected by fat feeding suggesting that the microsomal enzyme response to fat feeding was relatively specific.
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