Effect of Extraction Time on Tannin Antioxidant Level and Flavonoid on Pandan Wangi Leaf (Pandanusamary llifolius Roxb) Using Hydrothermal Extractor

Abstract

Pandanus leaf (Pandanus Amaryllifolius) is one of the plants that have chemical content in the form of tannins, saponins, polyphenols (phenolics), alkaloids and flavonoids that can be used as antioxidants.The research that is done is total tannin and alkaloid test on pandan leaves. The purpose of this reseach was to determine the effect of extraction time on the amount of tannin and alkaloids in pandan leaves. In the tannin and alkaloids test, the extraction of pandan leaves was first used hydrothermal extract with 60°C temperature in 60 minutes using aquadest solvent with variation of extraction time 20, 30, 40, 50 and 60 minutes. Tannin content of 0.2%; 0.8%; 2.5%; 6.2%; 7.1% and flavonoid levels of 2.05%; 2.36%; 4.44%; 0.78%; 0.34%. From the results of reseach showed that the longer the extraction time, the more tannin content produced. While flavonoids have optimum time at 40 minute extraction time. Optimal tannin content obtained by using aquades solvent is the time of extraction for 60 minutes, while the optimum flavonoid content at the time of extraction 40 minutes.