Effect of Extraction Solvents on Antioxidant Activity of Khejri (Prosopis cineraria L.) Stem Bark

Abstract

Studying the physiological effects of the three extraction solvents (water, methanol, and acetone) on the antioxidant activity of Khejri stem bark extract was the goal of this research (Prosopis cineraria L.). Khejri stem bark is characterized by their chemical and mineral composition. Higher phenol content, total flavonoid and antioxidant activity could be obtained using acetone as a solvent, followed by methanol and aqueous solution. The tested plant material contained a significant amount of total phenols (2.31 ± 0.13 mg GAE/g) and total flavonoids (0.88 ± 0.14 mg EC/g) in the acetone extract. With an increase in dynamically variable concentration levels, Khejri stem bark extract’s ability to scavenge DPPH free radicals rises. The total antioxidant capacity of the acetone extract was the greatest (1.180.89 mg AAE/g), and the acetone extract demonstrated the strongest free radical scavenging activity of DPPH and IC50 value was 179.44 mg/ml.