Abstract
The objective of the present study was to evaluate the effect of extraction solvents such as absolute ethanol, absolute methanol, aqueous ethanol (ethanol: water, 80:20 v/v), aqueous methanol (methanol: water, 80:20 v/v), hydro alcohol (ethanol: water 50:50 v/v) and water on phytochemicals of different parts of Aegle marmelos. The chemical comparison was done using thin layer chromatography. Major class of compounds such as phenolics and flavonoids were quantitatively estimated spectrophotometrically. Among the six solvents used for the extraction, hydro alcohol showed the maximum yield of extract. In the case of root, the yield of hydro alcoholic extract was 42.05 % higher than that of aqueous extract. The increase of yield for stem and fruit were found as 27.5 % and 46.35 % respectively. Due to the higher yield of extract, use of hydro alcohol instead of water for the preparation of herbal formulations can be considered and it may lead to the judicious use of raw materials.
Highlights
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Total flavonoid content (TFC) was expressed as mg quercetin equivalents
Folin-Ciocalteu reagent was procured from Sisco Research Laboratory (SRL), Mumbai, India, Gallic acid and quercetin were procured from Sigma Chemicals Co. (Bangalore, India)
Summary
Different Gallic acid standards were used for obtaining a standard curve. TPC was expressed as gallic acid equivalents (GAE) in mg / g of sample. Total flavonoid content (TFC) was measured by aluminium chloride colorimetric assay [14]. The flavonoid content was calculated from the calibration curve of standard quercetin. TFC was expressed as mg quercetin equivalents (mg EQ). The absorbance against the reagent blank was determined at 550 nm with UV-Visible spectrophotometer (Pharmaspec-1700, Shimadzu, Japan)
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