Effect of extracorporeal shock wave on osteogenesis-related and adipogenic-related genes of mesenchymal stem cells of patients with early or moderate stage osteonecrosis of the femoral head

Abstract

Objective To investigate the effect of extracorporeal shock wave (ESW) on osteogenesisrelated and adipogenic-related genes of mesenchymal stem cells (MSCs) of patients with early or moderate osteonecrosis of the femoral head (ONFH).Methods MSCs obtained from ONFH patients were divided into Group A and Group B.The proliferation was detected and compared.as well the content of alkaline phosphatase (ALP) in P_3,extracellular matrix mineralization in P_7,the expression of core-binding factor alpha 1 (Cbfα1),osteocalcin (OC) and peroxisome proliferator-activated receptor γ (PPARγ) between two groups at different time points.Results The proliferation of Group A was better than Group B significantly (except the 1st day);the ALP) content of Group A were higher than that of Group B significantly (except the 3rd day).The mineralized nodules was (6.0±1.0)/field in Group A,but negative in Group B.Cbfα 1 sustained expression in the whole process of ESW induced osteoblast differentiation,Group A was significantly stronger than the Group B every day (except the 3rd day);the OC expression of Group A was significantly earlier and stronger than that of Group B every day (except the 3rd,lay and 6th day).The PPARγ sustained expression in the whole process of ESW induced osteoblast differentiation,and that in Group A was significant stronger than that in Group B every day (exeept the 3rd day).Conclusion The appropriate intensity ESW can promote MSCs'proliferation,induce MSCs'differentiation into osteoblastic and inhibit MSCs'differentiate into fat cells,which may be an effective way of treating ONFH. Key words: High-energy shock waves; Femur head necrosis; Core binding factor alpha 1 subunit; Osteocalcin