Effect of external acetate added in aquaculture wastewater on mixotrophic cultivation of microalgae, nutrient removal, and membrane contamination in a membrane photobioreactor

Abstract

The mixotrophic cultivation of microalgae in wastewater has attracted extensive attention due to its many advantages. In this study, acetate, which can be prepared by hydrolysis of aquaculture waste, was used as exogenous organic matter to promote the growth of Chlorella pyrenoidosa cultured in aquaculture wastewater. Microalgae cultivation was carried out in a membrane photobioreactor (MPBR) with continuous inflow and outflow mode. The results showed that exogenous acetate greatly promoted the mixotrophic growth of C. pyrenoidosa. When the dosage of acetate reached 1.0 g L−1, the relative growth rate of microalgae in the logarithmic stage reached 0.31 d−1, which was 4.4 times that of the control. As a result, exogenous acetate also promoted the removal of nutrients from aquaculture wastewater. During the stable operation stage of the MPBR with acetate added in the influent, an average of 87.41%–93.93% nitrogen and 76.34%–88.55% phosphorus was removed from the aquaculture wastewater containing 19.41 mg L−1 total inorganic nitrogen and 1.31 mg L−1 total inorganic phosphorus. However, it was worth noting that adding exogenous acetate also led to an increase in the membrane resistance of the membrane module in the MPBR. Membrane resistance was mainly composed of internal resistance (Ri) and cake resistance (Rc), and with the increase of acetate content in the influent, their proportion in the total resistance gradually increased. Ri contributed the major membrane resistance and was most affected by acetate dosage. Ri reached 32.04 × 1012 m−1 with 1 g L−1 acetate, which accounted for 69.49% of total resistance. Moreover, with the increase of influent acetate concentration of the MPBRs, both the number of insoluble contaminants and dissolved organic contaminants in the membrane modules increased. In addition, the composition of proteins, polysaccharides, and humus in dissolved organic contaminants was close to that in extracellular polymeric substances and soluble microbial products secreted by microalgae. These results suggested that the membrane fouling of membrane modules was closely related to the algal biomass content in the MPBRs. The above results provided a theoretical basis for reducing membrane fouling of MPBR.