Abstract
This research was conducted in order to study the effect of explant type and some plant growth regulators on culture initiation of Stevia rebaudiana Bertoni in vitro. The experiments included surface sterilization and test two types of explants (shoot tips and stem nodes) and the impact of KIN and BA and IAA and IBA in the cultures initiation. Results revealed the efficiency of sodium hypochlorite (NaOCl) for disinfestation of explant at 0.050% concentration giving less contamination for shoot tips and stem nods (10% and 20% respectively). Results showed that shoot tips inoculated in MS medium plus KIN at 0.3 mg. L-1 was significantly increase the number of regenerated shoots as it produced 4.2 shoots per explant while medium without cytokinin (control) produced less number of shoots reached 1.4 shoots per explant. KIN treatment reduced shoots length as control treatment produced the highest length (6.74 cm). The interaction between the explant type and BA concentration was significantly increase the number of regenerated shoots as shoot tips produced 3.6 shoots per explant in MS medium supplemented with 0.1 mg. L-1. BA treatment reduced shoots length as control treatment produced the highest length (6.74 cm). No positive effect was gain when auxins (IBA and IAA) were added in combination with cytokinin in culture medium. The above results can be adopted to established stevia in vitro culture successfully.
Highlights
الع ارق لمموسم- محافظة ذي قار- قياس الكفاءة الاقتصادية والتسويقية لمحصول الخيار في البيوت البلاستيكية في الشطرة
Cucumber (Cucumis sativus L.) cucumber farmers in Dhi Qar province are is one of the most common vegetables in the making economic profits that enable them to world including Iraq despite its low nutritional expand their production
Greenhouses: This part of the study deals (5): with cost function analysis, which is an important economic criterion that measures the According to test (t), the estimated parameters economic and technical efficiency levels of were found to be significant at 1% and 5%, agricultural projects
Summary
Number of reasons; the most important of The total national production of cucumber which is the lack of optimal use of agricultural from these houses within this year was 156 resources. At the economic level of the country, production requirements, lack of experience these crops have a great value as they are an regarding the diagnosis and pest control, and important source of food for the essential low productivity compared to optimal volumes nutrients, and contribute to increase the of production, especially in greenhouses. Cucumber (Cucumis sativus L.) cucumber farmers in Dhi Qar province are is one of the most common vegetables in the making economic profits that enable them to world including Iraq despite its low nutritional expand their production. The analysis was done using the production of vegetables in greenhouses Eviews[10], Excel through facilitating agricultural loans Descriptive Analysis of the Cost specialized in this area of agriculture, as well Components of Cucumber Production in as the provision of many components of Greenhouses Fixed and variable costs for production such as fertilizers, seeds, covers, cucumber farms were analyzed to elucidate building structures, irrigation systems and each item in these costs (Table 1)
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