Abstract
ABSTRACT A study was conducted to evaluate the effects of exogenous enzymes supplementation in high protein (Hi-Pro) corn DDGS-based diets on broiler growth performance, intestinal health, and nutrient digestibility. A total of 200 one-day-old broiler chicks were randomly allocated to four dietary treatments with five replicates of 10 birds each. A basal diet (CON) was formulated (CP: 20%; ME: 2900 Kcal/kg) containing 15% of dietary crude protein share from Hi-Pro corn DDGS. Three experimental diets were developed using basal diets; diet one was supplemented with protease (CON-P), diet two with an enzyme mix of mannanase and xylanase (CON-MX) and diet three with a protease and enzyme mix of mannanase and xylanase. A digestibility assay was carried out using Celite (insoluble acid ash), from day 29 to day 35. On day 35, four birds from each replicate were picked randomly and killed by cervical dislocation to collect ileal digesta. In addition, duodenum, jejunum and ileum lengths were measured, and tissue samples were collected from midpoints of jejunum to note villus height and crypt depths. Feed intake, body weight gain and feed conversion ratio were not affected by dietary treatments during the entire study period (p>0.05). Likewise, no difference in carcass characteristics, soft organ weight, intestinal morphology, apparent amino acids digestibility and visible ileal digestible energy was observed by the dietary treatments (p>0.05).Supplementation of exogenous protease and enzyme mix (mannanase and xylanase) had no effect on growth performance, intestinal integrity and nutrient availability of broiler chickens fed Hi-Pro DDGS-based diets.
Highlights
Distiller dried grains with soluble (DDGS) is a corn by-product that is produced by dry milling and fermentation process
A tendency of improvement in body weight gain (BWG) was noted by the supplementation of exogenous enzymes during the starter phase over control
In another study Pedersen et al (2014) reported that NSP content of corn DDGS ranged 250-337 g/kg on DM basis and the distribution of sugar constituent was in the order of xylose >
Summary
Distiller dried grains with soluble (DDGS) is a corn by-product that is produced by dry milling and fermentation process. Ethanol producers are continuously improving on fractioning technologies to enhance the production of ethanol and the quality of DDGS (Jung & Batal 2009). Endosperm fraction of corn is subjected to fermentation in front end fractionation technology that eliminates non-fermentable fraction (germ and bran) of corn and results in (High-Pro) corn DDGS (Robinson et al 2008). High protein (Hi-Pro) corn DDGS is higher in crude protein (CP), amino acids and energy than conventional corn DDGS (Singh et al 2005). The inclusion of corn DDGS in broiler diet is well documented, higher level of corn DDGS has been reported to reduce growth performance (Lumpkins et al 2004; Wang et al 2007; Choi et al 2008; Youssef et al 2008; Loar et al 2010; Zhang et al 2013)
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