Effect of Exogenous Factors on Bacteriocin Production from Lactobacillus paracasei J23 by Using a Resting Cell System

Huaxi Yi,Yingchun Zhang,Yanyan Yang,Fang Ma,Wenli Liu,Hui Liu,Xue Han,Kai Sun,Lanwei Zhang

doi:10.3390/ijms141224355

Huaxi Yi, Yingchun Zhang + Show 7 more

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https://doi.org/10.3390/ijms141224355

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Abstract

A resting cell system was developed for bacteriocin Lac-B23 production from Lactobacillus paracasei J23. The resting cell medium contained (g/L): Glucose 20, Sodium acetate 5.0, MnSO4 0.25 MgSO4 0.5, Ammoniumhydrogencitrate 1.0, KH2PO4 1.0. The resting cell incubation time and temperature were 20 h and 37 °C and the effects of exogenous factors, including amino acids, glycerol, pyruvic acid, and α-ketoglutaric acid were investigated. Cys and Gly could stimulate the production of bacteriocin, while no stimulus effect was observed for Glu, Tyr and Ala. Glycerol and pyruvic acid increased bacteriocin production and the optimum concentrations were 1% and 30 g/L, respectively. Bacteriocin could act as an inducer of its own biosynthesis. These findings are of importance for the further study of bacteriocin biosynthesis regulation and for the improvement of bacteriocin production yields.

Highlights

Bacteriocins are ribosomally synthesized antibacterial proteins produced by different bacterial species [1,2,3]
Bacteriocin production can be induced by exogenous factors or exhibit auto-induction [19,20,21]
The composition of MRS has been optimized in preparation for a resting cell system [27]

Summary

Introduction

Bacteriocins are ribosomally synthesized antibacterial proteins produced by different bacterial species [1,2,3]. Bacteriocins produced by lactic acid bacteria (LAB) are attractive to the food industry because of their potential as natural food preservatives, and are generally recognized as safe (GRAS) [4,5]. A variety of LAB bacteriocins have been identified and characterized [6,7]. A potential bottleneck in LAB bacteriocin commercialization could be low production yields. It has been observed that the production of bacteriocin is influenced by the bacterial growth phase [9,10], medium composition [11,12,13,14], and culture conditions [15,16,17,18]. Bacteriocin production can be induced by exogenous factors or exhibit auto-induction [19,20,21]

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