Abstract
Aerial cultivation is a convenient method to propagate bulblets. To date, there are few studies on Lilium lancifolium (L. lancifolium) adventitious bulblet formation on bulb scales by aerial cultivation. We used the aerial culture method to study the process of exogenous 6-BA on bulblets formation. Through continuous observation of the bulblets formation process, the changes of endogenous hormones during the bulblets formation process and their correlation with gene expression were analyzed. In this study, we showed that exogenous 50 mg/L 6-BA was the most suitable concentration for bulblets formation in aerial cultivation which the bulblets formation rate was highest. Treatment with exogenous 50 mg/L 6-BA resulted in cell proliferation and a large aggregation of nuclei towards thin-walled cells, thus inducing the formation of bulblets and shorting the formation time of bulblets. The higher the ratio of iPA/ABA, DHZR/ABA, (iPA + DHZR + ZR)/ABA and (iPA + DHZR + ZR)/IAA were the better the induction of bulblets formation. qRT-PCR revealed that exogenous 50 mg/L 6-BA treatment induces bulblets formation by activating the expression of cytokinin receptors AHK2/3/4, activating ARR1/2/12 via AHP1, upregulating the expression of related genes involved in signaling and regulating downstream signaling.
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