Abstract
Abstract The concentration of cytochrome c has previously been shown to increase 2-fold in leg muscles of rats subjected to a program of prolonged exercise. In the present study it was found that the radioactivity of cytochrome c labeled with δ-amino[14C]levulinate decreased significantly more slowly in quadriceps muscles of rats subjected to a program of running than in muscles of sedentary animals. The t½ of muscle cytochrome c was 32 days in the sedentary animals and 48 days in the runners. The synthesis rate of cytochrome c was calculated to be approximately 0.630 nmole per muscle per day in the sedentary and 0.771 nmole per muscle per day in the exercised animals. Thus, a decrease in the rate of degradation plays the major role in accounting for the adaptive increase in the concentration of cytochrome c that occurs in muscle in response to exercise.
Highlights
The concentration of cytochrome c has previously been shown to increase Z-fold in leg muscles of rats subjected to a program of prolonged exercise
One protein of the mitochondrial inner membrane that increases in response to exercise is cytochrome c [3, 4]
We examined the effect of a program of exercise on the t,urnover of cytochrome c in leg muscles of rats to determine whether the increase in concentration is due to an increased rate of synthesis, a decreased rate of degradation, or a combination of the two
Summary
The concentration of cytochrome c has previously been shown to increase Z-fold in leg muscles of rats subjected to a program of prolonged exercise. Exercise can induce a 2-fold increase in some mitochondrial enzymes, while not affecting others (l-4), and there is electron microscopic evidence that both the size and number of muscle mitochondria increase in response to regularly performed exercise [5] These findings suggest that exercise could serve as a useful tool for studying the regulation of mitochondrial mass and composition, as well as the turnover of specific mitochondrial constituents. We examined the effect of a program of exercise on the t,urnover of cytochrome c in leg muscles of rats to determine whether the increase in concentration is due to an increased rate of synthesis, a decreased rate of degradation, or a combination of the two
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