Abstract
It was established that for in vitro rhyzogenesis of apple rootstock 106-13 it is better to use medium with 1/2 MS macro- and microsalts supplemented with IBA in concentration of 0.5 mg/l without use of etiolation (the yield of rooted microplants was 90.0 % with a coefficient of root system development – 1.14 ± 0.18). For in vitro rhyzogenesis of apple rootstock 54-118, it is better to use medium with 1/3 MS macro and microsalts, supplemented with IBA in concentration of 1.0 mg/l without etiolation (the yield of rooted microplants was 85.35 % with a coefficient of root system development – 0.53 ± 0.12). The positive effect of etiolation on root system growth depended on cultivar. For rootstock 54-118, the use of etiolation stimulated the growth of roots on medium with 1/3 MS macro- and microsalts combined with IBA in concentration of 1.0 mg/l (until the end of subculture). Use of etiolation inhibited the root system growth of rootstock 106-13 microplants until the end of subculture both on medium with 1/2 MS macro- and microsalts and on medium with 1/3 MS macro- and microsalts, but it stimulated root formation of rootstock 106-13. However, use of 7 days etiolation at stage of in vitro rhyzogenesis of rootstocks 106-13 and 54-118 is not expedient, because it does not stimulate the increase in the number of rooted microplants at the end of subculture.
Highlights
Use of 7 days etiolation at stage of in vitro rhyzogenesis of rootstocks 106-13 and 54-118 is not expedient, because it does not stimulate the increase in the number of rooted micro plants at the end of subculture
Этиоляция пролиферирующих культур при пониженных температурах в дальнейшем увеличивает укоренение побегов на среде с индолилмасляная кислота (ИМК)
Для ризогенеза in vitro подвоя 54-118 лучше использовать среду c 1/3 макро- и микросолей по MS, дополненную индолилмасляная кислота (ИМК) в концентрации 1,0 мг/л без этиоляции (выход укорененных растений-регенерантов – 85,35 %, коэффициент развития корневой системы – 0,53 ± 0,12)
Summary
При укоренении подвоя 106-13 на питательной среде с 1/2 макро- и микросолей MS лучше использовать ИМК в концентрации 0,5 мг/л без применения этиоляции (выход укорененных растений-регенерантов – 90,0 %, наилучший коэффициент развития корневой системы – 1,14 ± 0,18). 1. Влияние минерального состава среды на морфологические параметры подвоя 106-13 (через 6 недель культивирования, 0,5 мг/л ИМК без этиоляции) что при культивировании подвоя 54-118 на питательной среде с 1/2 макро- и микросолей MS на выход укорененных растений-регенерантов оказывали влияние концентрация ИМК (р < 0,001) и два фактора – концентрация ИМК и этиоляция одновременно (р < 0,001) – как после 3, так и после 6 недель субкультивирования.
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