Effect of ethyl acetate extract of usnea longissima on esophagogastric adenocarcinoma in rats1.

Renad Mammadov,Durdu Altuner,Elif Demirci,Emine Akyuz Turumtay,Hilal Alpcan,Halis Suleyman,Nihal Cetin,Adnan Yilmaz,Huseyin Baykal,Bahadir Suleyman

doi:10.1590/s0102-865020190030000005

Abstract

Purpose To investigate the effects of the EtOAc extract of U. longissima which is uninvestigated previously on esophagogastric cancer induced in rats with N-methyl-N-nitro-N-nitrosoguanidin (MNNG). Methods The anticancer activity of EtOAc extract of U. longissima was examined in the esophagogastric adenocarcinoma models induced in rats with MNNG. EtOAc extract of U. longissima, 50 and 100 mg/kg oral doses were administered once daily for six months. MNNG induced differentiated and undifferentiated type adenocarcinomas in the esophageal and gastric tissues of rats. Results EtOAc extract of U. longissima obtained from U. longissima prevented gastric and esophageal cancerogenesis induced in rats with MNNG. EtOAc extract of U. longissima did not have a lethal effect at doses of 500, 1000 and 2000 mg/kg. The prominent anticarcinogenic activity of EtOAc extract of U. longissima 50 and 100 mg/kg suggests that it is not toxic and it is selective to the cancer tissue. Conclusion This information may shed light on clinical implementation of EtOAc extract of U. longissima in future.

Highlights

In the treatment of cancer, different methods are used to reduce mortality and morbidity; such as surgery, radiotherapy, chemotherapy, immunotherapy, signal transduction inhibitors, gene therapy and angiogenesis inhibitors[1]
We investigated the effects of an ethyl acetate (EtOAc) extract of U. longissima on esophagogastric cancer induced by MNNG in rats
Ethyl acetate are mostly suitable for diffractaic acid, usnic acid and evernic acid wich were the majör compounds of U. longissima[12]

Summary

Introduction

In the treatment of cancer, different methods are used to reduce mortality and morbidity; such as surgery, radiotherapy, chemotherapy, immunotherapy, signal transduction inhibitors, gene therapy and angiogenesis inhibitors[1]. According to the recent statistics, more than 8 million people die from cancer every year[2]. This shows that modern medicine lacks effective curative options for the treatment of cancer patients. Lichens are not a single organism; they are symbiotic, composite organisms composed of fungi (ascomycetes, basidiomycetes) and photosynthetic algae[6]. The anticarcinogenic activity of a lichen type acid, usnea longissima, was investigated. The literature contains research on the effect of the extracts of different lichen types on gastric and esophageal cancer induced by MNNG. We investigated the effects of an ethyl acetate (EtOAc) extract of U. longissima on esophagogastric cancer (adenocarcinoma) induced by MNNG in rats. Ethyl acetate are mostly suitable for diffractaic acid, usnic acid and evernic acid wich were the majör compounds of U. longissima[12]

Methods

Results

Conclusion