EFFECT OF ETHIDIUM - BROMIDE ON ANTIBIOTIC RESISTANT OF UROPATHOGENIC E. COLI ISOLATES

Abstract

Antimicrobial Resistance among commonly –acquired uropathogens is an emerging concern over the past decades that warrants a continuing reevaluation of the appropraitens of recomended empiric antimicrobial Regimens for treatment of Urinary Tract Infections (U.T.I.s). Most of the Antibiotic Resistance Genes were plasmid determined, so it was, the first attempt to study the effect of curing agent (Ethidium-Bromide ) on Antibiotic Resistance of Uropathogenic E. coli isolates. (106) samples were collected from patients suffering from Urinary Tract Infection (U.T.I.). Samples were implanted on the culture media Eosinmethylene Blue (EMB) medium and MacConkey agar to isolate the bacteria and to diagnose them using microscopic, culture and biochemical tests and confirmed by the Vitck-2 system. Of the total, 45(42%) isolates were selected which belong to Escherichia coli. The susceptibility test towards eight antibiotics were carried out and the results showed that Ciprofloxacin, Erythromycin, Ampicillin, Norfloxacin, Ceftrixon and Amikacin were the most effective antibiotics and their resistance percentages were 20%, 20%, 20%, 20%, 30% and 30% respectively, Co-trimazole and Chloramphenicol were less effective and their resistance percentage were 90% both of them. Three isolates of E. coli (5,8,17) were selected depending on results of antibiotic sensitivity tests as showed multiple –antibiotic resistance (100%). First attempt made on the effect of Ethidium –Bromide (0.1%) as a curing agent on these three –multi-drug resistance (MDR) isolates which used at concentration (0, 10-1 , ------ -, 10-10 ) and the results showed E.coli (MDR) were sensitive to a ( Ciprofloxacin, Norfloxacin and ceftriaxone ) at Et.-Br. of concentration at (10-1 , 10-2 , 10-3 , 10-4 , 10-5 , 10-6 )while normal activity were observed at concenteation of (10-7 , 10-8 , 10-9 , 10-10 ) 0f Et.-Br. The results of Agarose – Gel Electrophoresis of both normal E. coli (MDR) and cured isolates showed the presence of chromosomal and plasmide DNA bands in the normal case while only chromosomal DNA bands with E. coli isolate no.(8) treated with an Ethidium –Bromide at concentration of (10-2 , 10-5 .