Effect of ethanolic seed extract of Caesalpinia bonducella on female reproductive system of albino rat: a focus on antifertility efficacy

Abstract

ObjectiveTo evaluate the effect of oral administration of the ethanolic seed extract of Caesalpinia bonducella on the reproductive system in Wistar female albino rat. MethodsThe study was divided into four groups. The group I received distilled water and served as vehicle treated control. Groups II, III and IV were orally administered 100, 200 and 300 mg/kg body weight dose of seed extract respectively, for 10 consecutive days and subsequently euthanized twenty four hours after the last dose. ResultsThe treatment prolonged the length of estrous cycle with significant increase in the duration of diestrus stage. The analysis of the principal hormones viz. LH, FSH, estradiol and progesterone showed significantly decreased levels in dose-dependant manner. Ovarian and uterine weight was significantly reduced as compared to that of the control group. Histoarchitectural observations revealed follicular atresia and degeneration of corpora lutea in ovary. Oviduct showed degeneration of mucosal folds and epithelium cells. Uterus showed evidence of degeneration of endometrial epithelium and endometrial glands. Lamina propria and muscularis layer of vagina were found slightly disorganized. ConclusionsIt can be concluded that significant decrease in the hormone levels, reproductive organs weight and alterations in histoarchitecture of reproductive organs might be due to antiestrogenic nature of seed extract.