Abstract
Jembrana diseases are caused by Jembrana Diseases Virus (JDV). The previous study showed that Jembrana Trans-Activator of Trancriptation (JTAT) recombinant protein is effective as a vaccine for Jembrana diseases. The production of JTAT protein needs to be optimized to obtain a higher amount of vaccine. High expression of JTAT protein will produce a high vaccine product. This study aimed to examine the effect of the addition of ethanol and IPTG in E. coli media on the expression of JTAT recombinant protein. This research was experimental research with factorial RAL design with a variation factor of ethanol and IPTG. Qualitatively, the induction of each IPTG, ethanol and interaction between the two could induce the expression of JTAT protein and could be identified with SDS-PAGE at ±11.8 kDa. Statistically, the induction of IPTG, ethanol and interaction between the two were not significantly different. Qualitative and quantitative data show that ethanol can induce JTAT protein expression. This result can be used as a preliminary study to test the effectiveness of ethanol as a substitute for IPTG in inducing the recombinant protein expression.
Highlights
Jembrana disease is a disease that only attacks Balinese cattle (Bos javanicus) (Kusumawati et al, 2015)
This study aimed to examine the effect of the addition of ethanol and isopropyl β-D-1thiogalactopyranoside (IPTG) in E. coli media on the expression of Jembrana Trans-Activator of Trancriptation (JTAT) recombinant protein
The source of the recombinant protein construct using the pBT7-C-His construction which was inserted by the tat gene as the JTAT protein-coding gene in the E. coli NiCo strain belonging to the LIPI Animal Molecular Genetics Laboratory
Summary
Jembrana disease is a disease that only attacks Balinese cattle (Bos javanicus) (Kusumawati et al, 2015). Jembrana disease generally attacks adult Balinese cattle with age ranged from 3-4 years (Mardiatmi, 2015). The agent that causes Jembrana disease is called Jembrana Disease Virus (JDV) (Kusumawati et al, 2014). Vaccines from recombinant DNA technology are used as an alternative to overcome the disease (Li et al, 2013). The advantages of recombinant-based vaccines include their ability to activate the humoral and cellular immune responses. DNA plasmids are produced in large quantities in a short time. Recombinant-based vaccines can increase the body’s immunity against viruses for a long time (Radji, 2009)
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