Effect of essential oils on the development of Colletotrichum sp. fungus in fragments of Feijoa sellowiana fruits

Adriana Terumi Itako,João Batista Tolentino Júnior,Amanda Do Prado Mattos,José Luis França Pinto Raduan,Karine Louise Dos Santos,Marlise Nara Ciotta

doi:10.4025/actascibiolsci.v43i1.53055

Abstract

The aim of this work was to evaluate the effect of the essential oils of Syzygium aromaticum, Cymbopogon citratus, Eucalyptus citriodora and Rosmarinus officinalis on the mycelial development of the fungus Colletotrichum sp. in fragments of Feijoa sellowiana fruits. The essential oils were incorporated in the PDA (Potato-Dextrose-Agar) medium in the concentrations of 250, 500 and 1000 ppm, 0 ppm (PDA only) (negative control), and fungicide fluazinam 1% (positive control). The area under the mycelial growth curve (AUMGC) and percent inhibition of mycelial growth (PIMG) were calculated. In the second evaluation, fruits fragments bordering the disease symptom were immersed in essential oils aqueous solution of S. aromaticum, C. citratus, and E. citriodora, at the concentration of 5000 ppm, 0 ppm (water only - negative control) and fluazinam 1% (positive control). The immersion times in the treatments were: 2, 4, 8, 12 and 24 hours, with subsequent incubation in Agar-Agar medium at 25°C. This evaluation was performed daily for 15 days, observing the moment of fungal germination through the emission of the mycelium. It was verified from the obtained results that all treatments reduced the fungal growth, and the essential oils of C. citratus and S. aromaticum totally inhibited its growth from the dose 500 and 1000 ppm, respectively. Regarding the test on fruit fragments, the essential oil of S. aromaticum at the immersion times of 12 and 24 hours was effective in inhibiting the fungus until the 15th day of evaluation.

Highlights

In Brazil, the abundance of autochthonous food species is substantial; this biodiversity is scarcely used compared to its potential
The experiment was conducted at the Phytopathology Laboratory of the Universidade Federal de Santa Catarina (UFSC), Curitibanos Campus
The response was dosedependent adjusted to the Gompertz model with 3 parameters, except for the essential oil treatment of R. officinalis which did not fit the model

Summary

Introduction

In Brazil, the abundance of autochthonous food species is substantial; this biodiversity is scarcely used compared to its potential. For this reason, it is the great relevance the encouragement of strategies aimed to increase the biological diversity, managing native species, as well as, to generate an alternative income. It is the great relevance the encouragement of strategies aimed to increase the biological diversity, managing native species, as well as, to generate an alternative income In this scenario, the South region of Brazil is home to several food species, among which Feijoa sellowiana O. The interest in the study of this myrtaceae is because this species is adapted to the edaphoclimatic conditions of south Brazil colder regions, and for presenting fruits with significant organoleptic potential (Ducroquet, Hickel, & Nodari, 2000)

Objectives

Methods

Results