Effect of essential oils of Artemisia aucheri Boiss. Zataria multiflora Boiss, and Myrtus communis L. on Trichomonas vaginalis

Abstract

Considering the high prevalence of Trichomonas vaginalis (TV) in women and the known side effects of metronidazol and giving importance to herbal drug therapy in order to reduce side effects in the recent decades, this study with the effect of essential oils of Artemisia aucheri Boiss., Zataria multiflora Boiss. And Myrtus communis L. in TV infection was done invitro condition This study has been carried out as double blind in test and control groups. The essential oils prepared by hydrodistilation. The parasite was isolated from vagina and determined directly. Samples were collected from vaginal discharge identification was done through direct smear parasite was added to the 5 test tubes containing dorsse medium, metronidazole dimethyl sulfoxaide (DMSO) and the essential oils with concentration of (o.1, 0.01, 0.001-0.004, 0.0002, 0.0001) in order to determine the effect of these concentration within 72 hour. Finding in this study showed that, Trichomonas could be alived in dorsse medium for 72 hours. In presence of metronidazol for one hour and in dorsse medium for 6 hours. The results revealed that, essential oil of Artemisia at concentration of 0.1, 0.01, and 0.001 are effective at the beginning of the inoculation and at concentrations of 0.0004 and 0.0001 after 1 and 2 hours respectively. Essential oil of Zataria at concentration of 0.1, 0.01, 0.001 and 0.0004 are effective at the beginning of the inoculation and at concentrations of 0.0002 and 0.0001 after 2 and 4 hours respectively Considering the significant effect of essential oils of Artemisia, zataria and myrtus on Trichomonas in in-vitro condition it is recommended that, the therapeutic effects the substance from this plant be studied in in-vitro condition and in case of having positive effect to be used as a drug