Effect of ERH gene on migration and invasion of human bladder cancer cells T24 and 5637

Abstract

Objective To study the effect of enhancer of rudimentary homolog (ERH) gene on migration and invasion in human bladder cancer T24 and 5637 cells. Methods After knocking out the ERH gene of human bladder cancer T24 and 5637 cells, Wound healing assay, Transwell cell migration assay and Transwell cell invasion assay were used to verify the migration and invasion function. Cell migration related protein was detected by Western blot. Nude mouse tail vein transfer assay was used to study the metastasis ability of bladder cancer cells in vivo. Results ⑴ The Wound healing assay showed that there were significant differences in the migration cell counts of human bladder cancer 5637 and T24 (P<0.05). ⑵ There were significant differences in migration and invasion cell counts of Transwell assay (P<0.05). ⑶ Western blot showed that the expression of E-Cadherin in human bladder cancer 5637 cells and T24 cells was significantly increased (P<0.05) after knocking out ERH gene, while the expression of Fibronectin, Twist, Vimentin and Snail2 protein were significantly decreased (P<0.05). ⑷ Nude mouse tail vein transfer assay showed that lung metastases were significantly reduced in the ERH knockout group compared with the normal ERH group. Conclusions Both in vitro and in vivo experiments suggest that ERH knockout affects the migration and invasion of human bladder cancer T24 and 5637 cells. Key words: Transcription factors; Cell cycle proteins; Urinary bladder neoplasms; Cell migration assays; In vitro