Abstract
In the present study we investigated the role of epidermal growth factor (EGF) in cholecystokinin-octapeptid (CCK8)-induced inositol 1,4,5-trisphosphate (IP3) production and amylase release. The data show that high EGF concentrations (90 nM) increased the basal amylase release, but did not increase the acinar IP3 content. High EGF concentrations shifted the dose-response curve for CCK8-induced amylase release to lower CCK8 concentrations, whereas low EGF concentrations (17 nM) shifted this dose-response curve to higher CCK8 concentrations. 17 nM EGF inhibited CCK8-induced IP3 production during the whole period of observation of 5 min, whereas 90 nM EGF inhibited only the initial component (15 s) of CCK8-induced IP3 production. At later time points, 90 nM EGF increased CCK8-induced IP3 production. We conclude that low concentrations of EGF are inhibitory on CCK8-induced IP3 production and amylase release, whereas high EGF concentrations stimulate CCK8-induced amylase release.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
