Effect of eosinophilopoetins on proliferative properties of blood eosinophil subtypes in allergic asthma

Abstract

<b>Introduction:</b> Blood eosinophil subtypes are different in biological properties. Supposedly various eosinophilopoetins can differentially affect eosinophil functions in asthma. <b>The aim:</b> To investigate interleukin (IL) 3, IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) effect on lung-resident-like eosinophil (rEOS-like) and inflammatory-like eosinophil (iEOS-like) promoted airway smooth muscle (ASM) cells proliferation in allergic asthma (AA). <b>Research methods:</b> We included 8 steroid-naive, non-severe AA patients and 6 healthy control subjects (HS). All AA patients underwent a bronchial allergen challenge with D. pteronyssinus and blood samples were recollected 24 h later. Eosinophils were isolated from peripheral blood by using high-density Ficoll centrifugation and magnetic separation methods. Phenotyping of eosinophils was based on magnetic beads-conjugated antibodies against L-selectin. ASM cells proliferation was assessed by the Alamar blue assay. <b>Results:</b> In AA group, IL-3 and IL-5 significantly enhanced rEOS-like cells proliferative properties, while GM-CSF had a significant effect on iEOS-like cells (p&lt;0.05). After bronchial allergen challenge, effect of GM-CSF on iEOS-like cells proliferative properties was further enhanced (p&lt;0.05). In HS group, only IL-5 had a significant effect on proliferative properties of iEOS-like cells (p&lt;0.05). <b>Conclusions:</b> IL-3 and IL-5 significantly enhanced proliferatives properties of rEOS-like cells, while GM-CSF had effect on iEOS-like cells in AA.