Effect of Environmental Parameters on hydrogen Production using &lt;i&gt;Clostridium Saccharoperbutylacetonicum&lt;/i&gt; N1-4(ATCC 13564)

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doi:10.3844/ajessp.2009.80.86

Abstract

Problem statement: Hydrogen gas production by Clostridium can be improved by several ways through media formulation, or suitable environment condition. This study was carried out to investigate the environmental factors effects on hydrogen production using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). Approach: The environmental factor studied includes initial substrate concentration, initial medium pH, temperature, sparging nitrogen and addition of Fe2+. Results: The result showed that the best yield of hydrogen produced (YP/S) was 3.10 moL (moL glucose)-1 when an initial glucose concentration was 10 g L-1, initial pH 6.0±0.2 at temperature 37°C. The volume of hydrogen produced was decreased when higher initial glucose concentration was applied. The yield of hydrogen increased when Fe2+ added to medium at concentration of 25 mg L−1. The yield and growth were further increased by sparging with nitrogen gas. Conclusion: It was observed that the best condition for highest hydrogen yield when initial pH 6.0±0.2 at 37°C and enhanced by adding ferrous sulfate in anaerobic process.

Highlights

Hydrogen gas has been suggested as a promising alternative to fossil fuels as an energy carrier for transportation needs
The culture stock was grown on Tryptone-Yeast Extract- Acetate (TYA) agar medium, Single colonies three to five of CS N1-4 from TYA agar culture were transferred anaerobically into 10 mL 15% Potato-Glucose (PG)
The best yield of hydrogen production on varying glucose concentration with time was 10 g L−1 as shown in Fig. 1b and this decreased with increasing glucose concentration

Summary

Introduction

Hydrogen gas has been suggested as a promising alternative to fossil fuels as an energy carrier for transportation needs. Shifting a fossil fuel economy to a hydrogen economy offers few environmental advantages if both are based on the net consumption of fossil fuels[1]. It is essential for reducing CO2 emissions that hydrogen production not release a net amount of CO2 into the atmosphere and that the technologies and materials used to produce hydrogen are sustainable. The production of hydrogen using mixed anaerobic bacterial cultures has attracted substantial interest recently due to its high conversion and non-polluting nature features as an ideal, clean and sustainable energy in contrast to chemical processes[1,2,3,4]

Methods

Results

Discussion

Conclusion