Abstract
The production of geosmin by axenic cultures of Fischerellamuscicola (ATCC 29114) was analysed by gas chromatography and mass spectrometry. A methanol based extraction procedure preceding closed loop stripping was successfully applied for quantitative analysis. The major part of geosmin produced by this cyanobacterium was bound in the cells. Less than 1% of the total geosmin was released. The content of geosmin in cells varied with culture conditions. Higher concentrations (based on protein amount) were observed in lag phase cultures and under anaerobic growth conditions. The synthesis of geosmin was temperature dependent showing minimum percentage concentrations at the optimum growth temperature (30°C). Heat shock treatment at 47°C did not affect the subsequent production of geosmin.
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