Effect of environment and shoot architecture on floral transition and gene expression in Eucalyptus occidentalis and Metrosideros excelsa

Abstract

Metrosiderosexcelsa and Eucalyptus occidentalis exhibit different strategies prior to flowering—the former passes through a long juvenile phase and must acquire a degree of architectural complexity to flower, whereas the latter flowers precociously even on stems still exhibiting juvenile foliage. As expediting flowering is of interest to breeders and horticulturalists alike we compared these species by growing plants with two branch architecture treatments in factorial combination with two growth environments. Plants were either allowed to branch freely or constrained to a single stem before subsequently being allowed to branch; one environment was inductive for flowering and the other not. Three meristem identity genes (the equivalents of LEAFY, APETALA1 and TERMINAL FLOWER1) were used as indicators of flowering. Constraining E. occidentalis plants to a single stem delayed the onset of the main flush of flowering in contrast to M. excelsa, although in both species a complex interaction between branching and environment occurred. We show that the complexity of the architecture can impact on production of flowers and can be used to expedite or enhance flowering for breeding purposes, but this is dependent on the species. AP1 appears to be a useful marker not just for floral organ differentiation but also as an indicator of floral induction having occurred.