Effect of enteral nutrition and probiotics on inflammatory factors and immune function in aplastic anemia rats

Abstract

Objective To investigate the effect of enteral nutrition and probiotics on inflammatory factors and immune function in aplastic anemia (AA) rats. Methods AA rats model were established by intraperitoneal injection of fluorouracil and lavage Maryland, and the successful AA rats model were randomly divided into model group, enteral nutrition group, probiotics group and combination group, 10 rats in each group. Another 10 Wistar rats were selected as blank group. Rats in the blank group and model group were given saline lavage (same volume 0.9% NaCl), rats in the enteral nutrition group were given Peptisorb nutrient solution [10 g/(kg·d)] by lavage, rats in the probiotics group were given live Clostridium butyricum [2 g/(kg·d)] by lavage, and rats in the combination group were given Peptisorb nutrient solution [10 g/(kg·d)] and live Clostridium butyricum [2 g/(kg·d)] by lavage for 4 weeks. The pathological structure of the bone marrow were observed by HE staining, the contents of white blood cells (WBC), red blood cell (RBC), platelet (PLT) and hemoglobin (Hb) in blood cell were detected by analyzer, the levels of serum tumor necrosis factor alpha (TNF-α), interleukin-2 (IL-2) and interleukin-11 (IL-11) were detected by ELISA method, and the contents of CD4+, CD8+ and NKT cells were detected by flow cytometry instrument. Results The contents of WBC, RBC, PLT and Hb in the model group were significantly lower than that in the blank group, the contents of WBC, RBC, PLT and Hb in enteral nutrition group, probiotics group and combination group were significantly higher than that in the model group, and the combination group was better than the enteral nutrition group and probiotics group (P 0.05). The level of TNF-α and IL-2 were significantly higher and the IL-11 level was significantly lower in the model group than in the blank group, the level of TNF-α and IL-2 were significantly lower and IL-11 level were significantly higher in the enteral nutrition group, probiotics group and combination group than in the model group, and the combination group was better than the enteral nutrition group and probiotics group (P 0.05). The contents of CD4+ and NKT cells were significantly lower and the CD8+ cells was significantly higher in the model group than in the blank group, the level of CD4+ and NKT cells were significantly higher and CD8+ cells were significantly lower in enteral nutrition group, probiotics group and combination group than in the model group, and the combination group was better than the enteral nutrition group and probiotics group (P 0.05). Conclusion Compared with normal consumption or oral glucose, the enteral nutrition combined with probiotics can reduce inflammatory response and improve bone marrow hematopoietic function and immune function in AA rats. Key words: Aplastic anemia; Enteral nutrition; Probiotics; Inflammation factors; Immune function