Effect of Entamopathogenic fungus, Nomuraea rileyi on the Larval Protein Patterns and Proteases Activity of Helicoverpa armigera (Hubner)

Abstract

The Helicoverpa armigera is one of the most serious polyphagus pest of many economically important crops. Nomuraea rileyi is an effective entomopathogenic fungus for the control of H. armigera has several advantages over other synthetic insecticide. N. rileyi LC50 concentration of 1.97×106 spores/ml was applied to the 4 th larval instar of H. armigera to investigate their impacts on the total protein and protease activity of body homogenate. Moreover, qualitative analysis of proteins in healthy developmental stages of H. armigera larvae and in N. rileyi treated larvae was detected using SDS-PAGE. The present investigation reported that there was difference in SDS-protein bands of control untreated and treated groups. The SDS-PAGE analysis of total body homogenate demonstrated that the few of proteins get under regulated upon treatment with N. rileyi. The quantitative analysis of total protein content and proteolytic activity was done. The H. armigera larvae upon treatment with N. rileyi produced significant decrease (p<0.05) in the total protein content of larval body while the proteases activity increased (p<0.05) significantly in N. rileyi treated larvae than the control larvae. The data of present findings help to understand a part how N. rileyi be effective in controlling H. armigera.