Effect of Enhanced UV-B Radiation on Arabidopsis Mesophyll Cell Protein

Abstract

Four-week-old wild-type Arabidopsis seedlings(Columbia-0) was treated using different doses of UV-B radiation,the protein in the mesophyll cells was extracted using acetone precipitation and TCA-acetone,then Arabidopsis mesophyllcellular protein content and composition of the different intensity of UV-B radiation response were analyzed.The results showed that comparing two methods,the protein content of the TCA-acetone extract is relatively higher,which was more suitable for the analysis of enhanced UV-B radiation on Arabidopsis mesophyll cell protein;The changes of protein contents by two extraction methods showed the same trend,along with the increasing of UV-B radiation dose,the protein content increased first and then reduced,B2 group reached a maximum.In addition,the number of protein bands and expression has taken place significant change,the most obvious changes were also in the middle dose treatment group(B2),both new bands and disappearance bands.This may be due to the Arabidopsis thaliana can activate some of its own resistance gene expressions and induce resistance protein by low doses of UV-B radiation,and thus resist the damage of UV-B;however,when subjected to high doses of UV-B radiation,damage their own protein synthesis pathway,and affect protein synthesis.