Abstract
The purpose of this study was to examine the effects of chronic exercise training (running 30 m/min, 10% grade, 90 min/d for 8-10 weeks) on specific renal enzyme activities involved with the gluconeogenic pathway in the fed and 24-hr fasted state in rats. A portion of the kidney (containing the cortex and medulla) was homogenized from which cytosolic (c) and mitochondrial (m) fractions were separated. Maximal gluconeogenic enzyme activities were assessed for: phosphoenolpyruvate carboxykinase (cPEPCK), fructose 1,6-bisphosphatase (cFBP), pyruvate carboxylase (mPC), aspartate aminotransferase (cAspAT), alanine aminotransferase (cAlaAT), and lactate dehydrogenase (cLDH). In the fed state, there was no significant difference between groups in any of the enzymes examined (nmoles/min x mg protein-1): cPEPCK (25.8+/- 1.7), cFBP (106.8+/- 7.1), mPC (20.7+/- 1.8), cAspAT (1047.1 +/- 38.6), cAlaAT (52.3 +/- 4.3), and cLDH (1728.6+/- 163.2). After the 24-hr fast, there was a significant increase in cPEPCK (52.4+/- 2.9 and 52.0 +/- 2.1) and mPC (44.6 +/- 4.3 and 47.6 +/- 4.9), control and trained, respectively. These results suggest that the maximal enzyme activities for cPEPCK and mPC can be augmented as a result of fasting that was independent of the training status.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International journal of sport nutrition and exercise metabolism
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
