Effect of Endostar combined with radiotherapy on tumor tissue perfusion and angiogenesis of Lewis lung cancer

Abstract

Objective To observe the effect of Endostar combined with radiotherapy on tumor tissue perfusion and angiogenesis of Lewis lung cancer.Methods The tumor size was measured and tumor growth curve drawn every other day,and contrast enhanced ultrasound wad used by directly injecting agent into tumor tissue.The software (SonoLiver 1.0) was used to obtain the time intensity curve of contrast analysis process (TIC) and dynamic vascular pattern curve (DVP).Results Tumor volume was increased with time,and the increasing rate in ES + RT group was obviously slower since D6 than other three groups (P ＜0.05).Differences in enhancement beginning time (s),time to peak (s),ascending slope (dB/t),enhanced strength of region of interest (dB),half time of descending (s) among time points showed statis-tical significance (P ＜ 0.05).The peak intensity (IMAX) and area under the curve (AUC) showed an increased trend with the time of tumor growth,but in the late stage declined changes occurred due to obvious necrosis in the tumor.Conclusion Endostar combined with radiotherapy could significantly inhibit the growth of tumor in mice with Lewis lung cancer and can influence the expression of tumor angiogenesis,improve the perfusion of tumor in mice.Contrast enhanced ultrasound can observe the change of tumor blood perfusion and necrosis area of the tumor,and exerts good reproducibility in quantifying the perfusion. Key words: Ultrasound imaging; Lung cancer; Blood perfusion; Endostar; Radiation therapy