Abstract
Poly lactic-co-glycolic acid (PLGA) has been widely investigated for various biomedical applications, such as craniofacial bone regeneration, wound dressing and tissue engineering. Electrospinning is a versatile technology used to produce micro/nanoscale fibers with large specific surface area and high porosity. The aim of the current study is to prepare PLGA nanofibers using electrospinning for guided tissue regeneration/guided bone regeneration applications. The objective of this study is to determine the appropriate electrospinning parameters such as applied voltage, flow rate, spinneret-collector distance and polymer solution concentration for preparation of PLGA fibrous membrane and their effect on the mean fiber diameter of the electrospun fibers. PLGA pellets were dissolved in Hexafluoroisopropanol (HFIP) in various concentrations overnight using a bench rocker. The resulting PLGA solution was then loaded into a syringe and electrospinning was done by maintaining the other parameters constant. Similarly, various fibrous mats were collected by altering the specific electrospinning parameter inputs such as applied voltage, flow rate and spinneret-collector distance. The morphology of the fibrous mats was characterized using Scanning Electron Microscope. The mean fiber diameter was assessed using ImageJ software and the results were compared using one-way ANOVA. We obtained bead-free uniform fibers with various tested solution concentrations. One-way ANOVA analysis demonstrated significant variation in mean fiber diameter of the electrospun fibers with altering applied voltage, solution concentration, flow rate and spinneret-collector distance. The above-mentioned electrospinning parameters and solution concentration influence the mean fiber diameter of electrospun PLGA nanofibers.
Highlights
With the advent of nanotechnology, functional polymeric nanofibers have emerged as a promising material in various biomedical applications such as tissue engineering, regenerative medicine, drug delivery, disease modeling and biosensing [1, 2]
The objective of this study was to evaluate the effect of electrospinning parameters and polymer solution concentration on the mean fiber diameter of the electrospun Poly lactic-co-glycolic acid (PLGA) nanofibers and to determine the appropriate electrospinning parameters and polymer solution concentration for the production of PLGA nanofibers to be used in the guided tissue regeneration/guided bone regeneration (GTR/GBR) membrane fabrication
The null hypothesis was that there was no significant difference in mean fiber diameter with altered electrospinning parameters and polymer solution concentration
Summary
With the advent of nanotechnology, functional polymeric nanofibers have emerged as a promising material in various biomedical applications such as tissue engineering, regenerative medicine, drug delivery, disease modeling and biosensing [1, 2]. Polymeric fibers prepared from natural polymers such as collagen, gelatin, chitosan, silk and alginate were largely investigated for tissue regeneration or repair because of their favourable characteristics as biocompatibility, biodegradability, and solubility in physiological environments [5,6,7,8,9,10,11,12,13,14,15,16,17,18] Their applicability was limited by immunogenic properties, unpredictable degradation rate, undefined release kinetics of loaded protein, poor mechanical properties, processing difficulties, cost, availability and the potential risk of transmitting animal-originated pathogens. The objective of this study is to determine the appropriate electrospinning parameters such as applied voltage, flow rate, spinneret-collector distance and polymer solution concentration for preparation of PLGA fibrous membrane and their effect on the mean fiber diameter of the electrospun fibers. Conclusion: The above-mentioned electrospinning parameters and solution concentration influence the mean fiber diameter of electrospun PLGA nanofibers
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