Effect of electroacupuncture on liver surface blood perfusion and related indexes of oxidative stress in mice with acute alcoholic liver injury

Abstract

To observe the effect of electroacupuncture (EA) on liver surface blood perfusion and related indexes of oxidative stress in mice with acute alcoholic liver injury （AALI）, so as to explore its mechanism underlying improving of AALI. Twenty-four Kunming mice were randomly divided into control, model and EA groups (8 mice/group). AALI model was established by intragastric administration of 50% ethanol at 6 g/kg. EA (4-6 V, 50 Hz) was applied to bilateral"Zusanli"(ST36) and"Taichong"(LR3) for 30 min. The liver surface blood perfusion was dynamically observed by moorFLPI laser speckle imager for 30 min. The morphological changes of the liver tissue were determined by H.E. staining. Levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in the liver were measured by ELISA. Compared with the control group, the model group had markedly pathological changes of the hepatic cords, hepatic sinuses and hepatocytes shown by H.E. staining, a significant reduction in liver surface blood perfusion and the content of SOD in liver tissue (P<0.01, P<0.05), and a significant increase in the content of MDA in liver tissue (P<0.05). Compared with the model group, H.E. staining showed reduced pathological changes of the liver tissue, meanwhile the liver surface blood perfusion significant increased (P<0.05) and the content of MDA in liver tissue significantly decreased (P<0.01) in the EA group. EA can improve the liver surface circulation of AALI mice, and regulate the oxidative stress response, and thus help to repair the damage of liver tissue.