Effect of electroacupuncture at “Zúsānlľ” ( ST 36) and other lower he-sea points on the expression of TNF-α and NF-κB of acute gastric mucosal lesion rats

Abstract

ObjectiveTo contrastively observe the effect of electroacupuncture at lower he-sea points of stomach, large intestine, small intestine and gallbladder of acute gastric mucosal lesion (AGML) model rats on the expression of serum high mobility group protein B 1 (HMGB 1), tumor necrosis factor-α (TNF-α) and nuclear factor-κB (NF-κB) of gastric tissue, and to explore whether the lower he-sea points of stomach have relative specificity in treatment of stomach diseases. MethodsSeventy healthy SD rats were randomly divided into seven groups, including control group, model group, “Zúsānlľ” (▪ ST 36) group, “Shàngjùxū” (▪ ST 37) group, “Xiàjùxū” (▪ ST 39) group, “Yánglíngquán” (▪. GB 34) group and “Chéngjīn” (▪ BL 56) group, with five male rats and five female rats in each group. Acute gastric mucosal lesion model was established by applying WRS method. After successful modeling and treatment for ten days, sampling was conducted, gastric mucosal lesion was observed visually and scored, the levels of serum HMGB 1 and gastric tissue TNF-α were detected by ELISA method, and the expression of NF-κB was detected by immunohistochemical method. Results▪ Compared with the model group, the levels of Ulcers and inflammation (Ul) in gastric mucosal and serum HMGB1, and the expression of gastric tissue TNF-α and NF-κB in the four lower he-sea points groups reduced (P<0.01 or P<0.05); (2) compared with ST 36 group, the levels of gastric mucosal Ul and serum HMGB1, and the expression of gastric mucosal TNF-α and NF-κB in other treatment groups increased significantly, and the difference was statistically significant (P<0.01 or P<0.05). Conclusion▪ Electroacupuncture at relevant lower he-sea points can inhibit the body's immune response, relieve inflammatory reaction and reduce mucosal lesion by regulating HMGB 1, TNF-α and NF-κB, so as to realize the intervention and treatment on AGML rats; ▪ the total intervention effect in ST 36 group is superior to that in other groups, indicating partially that there is a relative specificity between ST 36 and stomach.