Abstract
To evaluate the effect of EGF in primary culture of ulcer patients and N87 cell line on expressions of apoptotic genes. Ulcer patients who applied to Gastroenterology Clinic of Mersin University Medical Faculty were included in this study. EGF application to the primary culture of biopsy samples with ulcer patients and gastric cancer cell-line (ATCC-NCI-N87) has been carried out at three different doses. The expression levels of Bax, Bcl-2 and Fas genes were measured with Quantitative Real-Time PCR (qRT-PCR). ΔΔCT analysis with qRT-PCR revealed no significant change in gene expression of Bax, Bcl-2 or Fas within the ulcer, normal tissue and gastric cancer. No significant change was determined between Bax and Bcl-2 gene expression levels and applied EGF doses when groups were compared for each EGF dose. On the other hand, when 50 ng/µl of EGF was administered, Fas mRNA expression level was significantly lower in gastric cancer cell line compared to patients with ulcer and normal gastroduodenal tissue (p ˂ 0.05). In this study which was done with a restricted patient group, our results revealed that apoptosis induced by Fas expression in gastroduodenal suppressing carcinogenesis process plays an active role in gaining anti-apoptotic properties of cells.
Highlights
The gastric mucosa is normally exposed to a wide range of aggressive factors and has developed efficient mechanisms to repair tissue injury
In this study which was done with a restricted patient group, our results revealed that apoptosis induced by Fas expression in gastroduodenal suppressing carcinogenesis process plays an active role in gaining anti-apoptotic properties of cells
The aim of the present study is to analyze the effect of Epithelial Growth Factor (EGF) in ulcer patients on expressions of genes related to apoptosis (Bax, Bcl-2, Fas), and show, if any, the similarities and/or differences among expression patterns in the cell line
Summary
The gastric mucosa is normally exposed to a wide range of aggressive factors and has developed efficient mechanisms to repair tissue injury. In the case of deeper mucosal damage, such as erosions and ulcers, both epithelial and connective tissue components, including sub-epithelial myofibroblast, smooth muscle cells, vessels, and nerves are destroyed and must be regenerated. Polypeptide growth factors such as Epithelial Growth Factor (EGF) have received much attention in recent years because of their ability to regulate essential cell functions involved in tissue healing including cell proliferation, migration, differentiation, secretion [1]. EGF is synthesized by submandibular glands, Brunner’s glands of the duodenum, and Paneth cells of the small intestine, but not in the normal gastric mucosa. Salivary levels and gastric fluid concentrations of EGF are reduced during the active phase of peptic ulcer disease, but are restored to normal levels after ulcer healing is complete
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