Effect of EGCG on SGC‐7901 cells migration and metastasis

Abstract

Objective: The actin binding protein Vasodilator‐stimulated phosphoprotein(VASP) has complex effects on cytoskeletal organization and cell motility, which play a positive role on tumour cells migration and invasion. Our research aim to investigate the effect of epigallocatechin gallate (EGCG), an extractent from green tea, on SGC‐7901 cells migration and invasion. Methods: The expression level of VASP in SGC‐7901 cells was detected by RT‐PCR and Western Blotting. Down regulation of VASP expression was performed with VASP siRNA transfection, while the activation of Rac1 pathway was enhanced by fMLP. Wound‐healing assay (2D assay) and transwell migration assay (3D assay) were employed to analyze invasive migration ability of SGC‐7901 cells. Results: After treated with EGCG, the expression level of VASP in SGC‐7901 cells decreased significantly at mRNA and protein level compared with control group (P<0.05). In 2D assay, the cell migration velocity was reduced by EGCG (P<0.05), accordingly the EGCG group showed lower invasion capacity than the control group in 3D assay (P<0.05).The effect of EGCG on SGC‐7901 cells migration and invasion was enhanced by VASP siRNA transfection, on the contrary decreased by fMLP. Conclusion: EGCG has a inhibitive effect on SGC‐7901 cells migration and invasion by decreasing the expression level of VASP via Rac1 pathway. (This work was supported by National Natural Sciences Foundation of China under Grant No.30770966)