Effect of ectopic splitting combined with portal vein ligation on rat liver regeneration

Abstract

Objective To explore the effect of ectopic splitting combined with portal vein ligation on rat liver regeneration. Methods Sixty Sprague-Dawley rats were randomly divided into four groups (n=15): portal vein ligation group (PVL group), associating liver partition with PVL group (ALPP group), associating spleen partition combined with PVL group (ASPP group) and associating kidney partition combined with PVL group (AKPP group). Hepatic regeneration rate (HRR), ALT, AST and hepatocyte proliferating cell nuclear antigen (PCNA) were detected on the 2nd, 4th and 7th day after operation. Results On the 4th and 7th day, in ALPP group, ASPP group and AKPP group, there was an obvious hyperplasia (F=3.750, P<0.05; F=16.398, P<0.01) in the right middle lobe of the liver and higher PCNA (F=6.860, P<0.01; F=3.810, P<0.05) in the liver tissue than in PVL group. And ALPP group had significantly higher ALT and AST level on the 2nd and 4th day (ALT: F=8.812, F=21.929; AST: F=36.393, F=6.468; P<0.01). Conclusions Ectopic splitting combined with PVL can significantly promote rat′s liver regeneration, possibly by inflammatory response, stress response and high expression of certain cytokines. Key words: Liver regeneration; Portal vein; Cytokines