Abstract
In this study, water-insoluble fraction of edible birds' nest (EBN) was treated with dynamic high pressure microfluidization (DHPM) under 40, 80, 120, and 160 MPa of pressure. The results displayed a remarkable pressure-related increase in protein solubility upon DHPM treatment. In addition, circular dichroism spectra also revealed that DHPM treatment induced conformational changes such as increase in α-helix and β-turn, whereas intrinsic fluorescence spectroscopy showed that tryptophan residues were less exposed to solvent. Furthermore, the increased surface hydrophobicity accompanied by reduced particle size was observed in DHPM treated samples, which was in good agreement with results of atomic force microscopy analysis, showing de-aggregation of large proteins in EBN water-insoluble fraction. Hence, our study shows that DHPM treatment can solubilize proteins from EBN water-insoluble fraction, and is an alternative method to traditional stewing to release the functional ingredients from EBN insoluble fraction, which can be introduced into industrial EBN food processing.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
