Abstract
Objective: Reperfusion injury occurs when the condition causing ischemia in ovarian torsion is corrected and the blood supply is re-established. The aim of this study is to evaluate whether doxycycline treatment reduces reperfusion injury in a rat model.Study Design: Thirty-five female albino Wistar rats were split into the five groups. Sham: Sham operation; ischemia (I): 2 hours of ischemia; ischemia and reperfusion (I/R): 2 hours of ischemia followed by 2 hours of reperfusion; Sham-Dc: Sham operation and doxycycline 10-mg/kg (2 hours prior to surgery); I/R-Dc: 2-hours of ischemia and doxycycline 10-mg/kg and 2-hours of reperfusion. The groups were compared in terms of histological and biochemical features. A semi-quantitative histological assessment scoring system was used for the histological examination. Follicular cell degeneration, vascular congestion, haemorrhage and inflammatory cell count were evaluated for histological analysis. For biochemical analysis of reperfusion injury, the body's total antioxidant status and total oxidant status were measured using a fully automatic method. The oxidative stress index was calculated by dividing total antioxidant status by total oxidant status.Results: In the sham group the ovaries were histologically normal. Oedema, vascular congestion, bleeding, leukocyte infiltration and follicle degeneration were increased in other groups (p<0.05). There was less leukocyte infiltration in the I/R-Dc group compared to I/R group. Other histological features were similar in these groups. Doxycycline increased the malondialdehyde, total antioxidant and total oxidant status levels in Sham-Dc and I/R-Dc groups. This increase was statistically significant between Sham and Sham-Dc groups. However, although there was an increase in the biochemical markers in the I/R-Dc group, this increase was not significant compared to I/R group.Conclusion: Doxycycline treatment in ovarian torsion does not reduce I/R injury. Doxycycline may even increase reperfusion injury, according to biochemical findings.
Highlights
Ovarian torsion is a partial or complete rotation of the ovary that disrupts its blood flow around the ligamentous connections
Doxycycline increased the malondialdehyde, total antioxidant and total oxidant status levels in sham group (Sham)-Dc and ischemia and reperfusion (I/R)-Dc groups. This increase was statistically significant between Sham and Sham-Dc groups
There was an increase in the biochemical markers in the in rats given doxycycline (I/R-Dc) group, this increase was not significant compared to I/R group
Summary
Ovarian torsion is a partial or complete rotation of the ovary that disrupts its blood flow around the ligamentous connections. It is one of the most common gynaecological emergencies for all age groups [1,2]. Ischemia that develops secondary to impaired blood flow causes loss of ovarian reserve. Diagnosis and surgical correction to restore blood flow are very important. Reperfusion injury is the damage that occurs when blood flow is restored to under-oxygenated tissues. Events occurring during reperfusion of ischemic tissue lead to increases in mediators such as TNF-alpha, interleukin-1, platelet activating factor, and nitric oxide [4]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
