Abstract
Objective To investigate the effects of down-regulation of integrin-β1 expression on cell invasion and chemotherapy-resistance of human colon carcinoma cell line HT-29. Methods The down-regulation of integrin-β1 mRNA expression by antisense oligonucleotide technologies was determined with reverse transcription-polymerase chain reaction (RT-PCR). The invasive ability in vitro was evaluated by Transwell invasion chamber assay. Growth inhibition rates and apoptosis rates of HT-29 cells under different dosages of chemotherapeutic agents [5-fluorouracil (5-Fu)] were measured by methyl thiazol tetrazolium (MTT) colorimetric assay and flow cytometry (FCM). Results In contrast with control group,the expression of integrin-β1 mRNA of HT-29 cells were markedly down-regulated,and the number (59 ±12) of migrated HT-29 cells was significantly decreased in the experimental group (P ＜ 0. 05 ). Higher dosages ( 100 mg/L and 200 mg/L of 5-Fu) caused a greater increase of inhibition in experimental group than in control ( P ＜ 0. 05 ). Conclusion The results demonstrate that down-regulation of integrin-β1 expression can effectively decrease cell invasion and chemotherapy-resistance of human colon carcinoma cell line HT-29. Key words: Cell invasion; Chemotherapy-resistance; Colon carcinoma; Integrin-β
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