EFFECT OF DOSED NORMOBARIC HYPOXIA ON MORPHO-FUNCTIONAL CHANGES IN HEPATOCYTES OF NORMOTENSIVE AND HYPERTENSIVE RATS

Abstract

Problem. Study of the effect of dosed hypoxia on the hepatocytes activity and morphometric parameters of rats of different lines.The aim of the work was to investigate and compare the morphological and functional changes in hepatocytes of normotensive and spontaneously hypertensive rats after exposure to dosed normobaric hypoxia.Materials and methods. The study was conducted in the autumn on 48 male rats of the Wistar and SHR line. Experimental animals were given daily hypoxic gas mixture (12 % oxygen in nitrogen) in intermittent mode: 15 minutes deoxygenation / 15 minutes reoxygenation for 2 hours. The duration of the experiment was 28 days. Histological preparations were made from liver tissue using a standard technique. Morphometry was performed using the computer program "Image J". The activity of succinate dehydrogenase and protein concentration in a hepatocytes mitochondrial suspension was determined.Results. It was revealed that morphological changes in the liver parenchyma and biochemical parameters of hepatocyte mitochondria activity in spontaneously hypertensive rats, breathing in a hypoxic gas mixture, were manifested to a greater extent than in animals of the Wistar line. Experimental animals showed an increase in the number of binuclear cells (SHR line) and nucleolus in the nucleus hepatocyte, an increase in nuclear-cytoplasmic and nucleolus-nuclear ratio, as well as an increase in succinate dehydrogenase activity and protein concentration (SHR line) in a suspension of hepatocyte mitochondria. These data may indicate an increase in the functional activity of hepatocytes and activation of physiological cell regeneration at the intracellular level.Conclusions. The effects of dosed normobaric hypoxia have morphofunctional signs of increased physiological regeneration and activity of the liver parenchyma in spontaneously hypertensive rats to a greater extent than in animals of the Wistar line.Key words: liver; intermittent hypoxia; arterial hypertension.