Effect of dobutamine on biological behavior and expression of YAP protein of human lung cancer cell line A549 treated with cis-diamminedichloroplatinum

Abstract

Objective To investigate the effect of dobutamine on biological behavior and the expressions of YAP and phospho-YAP (P-YAP) of human lung adenocarcinoma A549 cells treated with cis-diamminedichloroplatinum (DDP). Methods Human adenocarcinoma A549 cells were cultured and divided into control group, dobutamine group, DDP group and dobutamine+DDP group. The proliferation inhibitory rates of these groups were detected by CCK-8 assay. The invasive abilities of these groups were detected by Transwell method. The scratch healing experiment was used to test the migration and movement capacity of the cells. The apoptosis rates of these groups were analyzed by flow cytometry. The protein expressions of YAP and P-YAP of these groups were determined by Western blot. Results Dobutamine or DDP could inhibit the proliferation of A549 cells in a dose-dependent and time-dependent way (P < 0.05). In control group, dobutamine group, DDP group and dobutamine + DDP group, the scratch healing rates were (41.22±0.70) %, (16.11±0.39) %, (14.49±0.25) % and (7.24±0.30) %, respectively (P < 0.05), the transmembrane cell numbers were (262.91±2.81), (115.03±4.26), (81.88±3.91) and (48.51±3.99), respectively (P < 0.05), and the apoptosis rates were (22.28±0.61) %, (27.92±1.36) %, (32.16±1.80) % and (36.70±1.87) %, respectively (P < 0.05). The combination of dobutamine and DDP could inhibit the expression of YAP and promote the expression of P-YAP compared with dobutamine group (P < 0.05). Conclusion Dobutamine may enhance the DDP effect by descending the expression of YAP to restrain the proliferation, invasion and migration, and to enhance the apoptosis of A549 cells. Key words: Lung adenocarcinoma; Dobutamine; Cisplatin; Yes-associated protein