Effect of Dissolved Oxygen Concentration on Growth of Mouse-Mouse Hybridoma

Abstract

The effects of the dissolved oxygen concentration (DO) in the culture broth on the cell growth, consumption rates of nutritional elements such as glucose and amino acids, and production of anti-IgE antibody, lactic acid and ammonia were studied. Four batch cultures were carried out under the different DO conditions, 75, 50, 20 (corresponded to air saturation, ca.7 ppm) and 6%. With the increase of DO, maximum values of viable cell number and antibody produced decreased, but the amount of ammonia produced was almost constant. The specific growth rate was almost constant below 50% of DO, but at 75% of DO decreased remarkably. The specific production rates of inhibitory compounds, ammonia and lactic acid were minimum at 6% of DO. Cell yield from glucose or glutamine was maximum at 6% of DO, and decreased with the increase of DO. In conclusion, DO level at 6% seemed to be favorable for the effective antibody production by the hybridoma cells.