Abstract
This work evaluated the effect of dissolved oxygen and the initial inoculum concentration on xylose reductase (XR) production by Candida guilliermondii from sugarcane bagasse hemicellulosic hydrolysate. Both the parameters were studied under an experimental design 22 with triplicate at central point. The statistical analysis of the results indicated a significant negative effect on XR production from the variable inoculum. The variable dissolved oxygen also showed a negative effect on XR production. We found the maximum enzyme activity (2.5 U?mg?1) when both the factors were applied at their lowest levels. The yeast showed to be potentially capable for xylose reductase production when sugarcane bagasse hemicellulosic hydrolysate was used as carbon source. Also, the results presented important information for further optimization of xylose reductase attainment.
Highlights
D-xylose is the most abundant five carbon sugar in nature and is one of the major constituent mono- saccharide in plant cell wall [1,2]
This work evaluated the effect of dissolved oxygen and the initial inoculum concentration on xylose reductase (XR) production by Candida guilliermondii from sugarcane bagasse hemicellulosic hydrolysate
The yeast showed to be potentially capable for xylose reductase production when sugarcane bagasse hemicellulosic hydrolysate was used as carbon source
Summary
D-xylose is the most abundant five carbon sugar in nature and is one of the major constituent mono- saccharide in plant cell wall [1,2]. Inoculum concentration and dissolved oxygen levels are the two most important factors which influence the optimum XR production from a variety of microor- ganisms [14,15,16]. Oxygen supply affects the rate and yield of xilitol and ethanol which determines the portioning of the carbon flux from xylose between cell growth and product formation [17]. 236 Effect of Dissolved Oxygen and Inoculum Concentration on Xylose Reductase Production from Candida guilliermondii Using Sugarcane Bagasse Hemicellulosic Hydrolysate phate dehydrogenase production from C. guilliermondii, which is an intracellular enzyme. Inoculum level and the dissolved oxygen were studied in order to verify their effect on XR production by the yeast C. guilliermondii FTI 20037 using sugarcane bagasse hemicellulosic hydrolysate under an experimental design 22 with triplicate at central point
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