Abstract
The in-vitro rumen fermentation study was conducted to evaluate the effect of supplementation of direct-fed microbial (DFM) in the total mixed ration (TMR) on total gas production, methanogenesis and dry matter digestibility by using rumen liquor of adult Surti buffaloes. DFM was supplemented at 0, 1, 2, 3, 4, 5, 6, 7, and 8% with TMR (65% Straw and 35% concentrate) for in-vitro gas production trials. The result of the in-vitro study revealed significantly (P<0.05) higher IVDMD (57.03%) and lower CH4 production (3.20 ml CH4/100 mg DDM) at a 3% level of DFM supplementation in TMR. Based on the overall results of in-vitro studies 3% level of DFM supplementation was found to be most suitable for further in-vivo studies in adult Surti buffaloes.
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